Composite

Part:BBa_K3440010:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


luxI-Myc under bphR1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 963
    Illegal BglII site found at 1001
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


Design Notes

This part was designed to show that LuxI can be expressed under induced bphR1 promoter, and compare expression with/without PCB. Myc tag was added to ease characterization. BBa_B0034 was used as an RBS because it is a moderate strength RBS.

Source

LuxI from Vibrio fischeri

References